DigesTip proteinase K, 10 ul, 8 tips per pack

DigesTip proteinase K is a micro-bioreactor shaped as a pipette tip with a filter containing immobilized proteinase K.  DigesTip proteinase K exhibits unprecedented great performance in terms of ease of use, digestion time and quality of results. It is unprecedentedly effective on native proteins and a powerful resource for rapid inactivation of endogenous RNase and DNase during nucleic acid isolation. DigesTip proteinase K is compatible with the majority of 20 µl and 200 µl single-channel and multi-channel pipettors, including: Gilson, Biohit, Rainin, Eppendorf, Brand.

Technical facts
Eukaryotic cell lysate
Lysis buffer: 10 mM Tris, pH 8.5,
5 mM EDTA, 200 mM NaCl, 0.2% SDS
DigestTip digestion time: 1 minute

Lane A) Protein extract from 10E6 BTC-1 cells in 1 ml of lysis buffer.
Lane B) Protein extract digested with proteinase K at a concentration of 140 µg/ml at 60°C for 1 hour.
Lane C) Protein extract digested with DigesTip Proteinase K at room temperature for 1 minute.
Lane D): Molecular Weight Standard.

Technical facts
Hybridization
Lysis buffer: 50 mM tris pH 7.5, 5 mM EDTA, 200 mM NaCl, 1% SDS.
Southern blot analysis. Murine stem cells were solubilised with lysis buffer and digested with proteinase K or DigesTip proteinase K. After digestion, genomic DNA was extracted with the phenol/chloroform method, digested with EcoRI and separated on agarose gel, then transferred to a nylon filter and hybridized with 32P–specific probe.

Lane A) Sample digested overnight with Proteinase K, 300 µg/ml, at 55°C.
Lane B) Sample digested with DigesTip Proteinase K for 1 minute at room temperature.
Lane C) Negative control.