The Green DNA provides an easy 2-step method to stain DNA bands from DNA electrophoresis. This unique reagent ensures DNA to be stained with a high sensitivity and good quality. Green DNA is a next-generation DNA-binding dye with features ideal for use in quantitative real-time PCR (qPCR) and many other applications. The dye was designed by taking into consideration several essential dye properties relevant to PCR, including PCR inhibition, safety, and stability and fluorescence spectra of the dye. Ethidium bromide (EtBr), which can detect 1-5 ng of the double-stranded DNA (dsDNA) in the agarose gel analysis, has been the most common dye used for nucleic acid gel staining. However, several drawbacks of EtBr have been understood, including that EtBr is a mutagen/carcinogen and presents a high risk of inducing cancer. Moreover, the ultraviolet(UV) light used to illuminate EtBr-DNA compounds will probably result in skin or eye damage to the user if misconducted. It’s also noted that exposure to the UV light might cause chemical modifications of the DNA samples in the gel, such as the formation of TT dimers, leading to challenges with the subsequent DNA manipulations. Reduced efficiency of transformation was observed by our scientists, after conducting ligation with the DNA samples isolated from the gel exposed to a longer period of UV illumination.
As compared with the EtBr, the Green DNA shows a much higher sensitivity under the 254 nm transillumination and is one of the most sensitive stains for detecting dsDNA in the agarose gel. In addition to the high sensitivity, the Green DNA brings a more reliable and safer user experience since the stained gel can be visualized with the blue-light transilluminator, thus avoiding the risk of skin/eye damage as well as reducing the side effects of DNA modification caused by the UV light.
Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash.
Ultra-sensitive: More sensative than SYBR Green I
Flexible for different procedures: Can be used for either precast or post gel staining.
Simple to use: Very simple procedures for precast and post gel stainings.
Perfect Compatibility with a Standard UV or a Blue light Transilluminator: Green DNA replaces EtBr with no optical setting change; Green DNA replaces SYBR Green I with no optical setting change.
1KB DNA Ladder (250-10k bp) was 2X serial diluted (from 2 to 128 dilution, respectively) and loaded in the 1% agarose gel. After electrophoresis, the gel was stained for 10 min with Green DNA (Fig. 1a) or with SYBR® Green I from another Brand (Fig. 1b). The left stained gels were observed with the UV 254 transilluminator and photographed by CCD camera, and the right stained gels were observed with the BlueLight transilluminator.
MSDS (66.55 kB)
Protocol (44.82 kB)